Plant Transcription Factor Database
Previous version: v3.0
Ocimum tenuiflorum
HSF Family
Species TF ID Description
Ote100002990061HSF family protein
Ote100005190011HSF family protein
Ote100022550211HSF family protein
Ote100025380051HSF family protein
Ote100034290001HSF family protein
Ote100039230111HSF family protein
Ote100049860041HSF family protein
Ote100057290021HSF family protein
Ote100068500151HSF family protein
Ote100070710161HSF family protein
Ote100071490011HSF family protein
Ote100071740091HSF family protein
Ote100087380061HSF family protein
Ote100106210061HSF family protein
Ote100119640021HSF family protein
Ote100127330141HSF family protein
Ote100133910091HSF family protein
Ote100143260091HSF family protein
Ote100176040041HSF family protein
Ote100183290261HSF family protein
Ote100183920021HSF family protein
Ote100185550061HSF family protein
Ote100185850181HSF family protein
Ote100190050001HSF family protein
Ote100216000021HSF family protein
Ote100227020121HSF family protein
Ote100237610011HSF family protein
Ote100254010181HSF family protein
Ote100258130031HSF family protein
Ote100258130121HSF family protein
Ote100258710081HSF family protein
Ote100266890031HSF family protein
Ote100273220021HSF family protein
Ote100274510061HSF family protein
Ote100277030081HSF family protein
Ote238038890041HSF family protein
HSF Family Introduction

Heat stress transcription factors (Hsfs) are the major regulators of the plant heat stress (hs) response. Sequencing of the Arabidopsis genome revealed the existence of 21 open-reading frames (ORFs) encoding putative Hsfs assigned to classes A-C. Here we present results of a functional genomics approach to the Arabidopsis Hsf family focused on the analysis of their C-terminal domains (CTDs) harboring conserved modules for their function as transcription factors and their intracellular localization. Using reporter assays in tobacco protoplasts and yeast as well as glutathione-S-transferase (GST) pull-down assays, we demonstrate that short peptide motifs enriched with aromatic and large hydrophobic amino acid (aa) residues embedded in an acidic surrounding (AHA motifs) are essential for transcriptional activity of class A Hsfs. In contrast to this, class B and C Hsfs lack AHA motifs and have no activator function on their own. We also provide evidence for the function of a leucine (Leu)-rich region centered around a conserved QMGΦL motif at the very C-terminus as a nuclear export signal (NES) of class A Hsfs. Sequence comparison indicates that the combination of a C-terminal AHA motif with the consensus sequence FWxxF/L,F/I/L as well as the adjacent NES represents a signature domain for plant class A Hsfs, which allowed to identify more than 60 new Hsfs from the expressed sequence tag (EST) database.

Kotak S, Port M, Ganguli A, Bicker F, von Koskull-Doring P.
Characterization of C-terminal domains of Arabidopsis heat stress transcription factors (Hsfs) and identification of a new signature combination of plant class A Hsfs with AHA and NES motifs essential for activator function and intracellular localization.
Plant J, 2004. 39(1): p. 98-112.
PMID: 15200645